Live-Cell Imaging


Please contact Hongyu Noel to arrange an appointment to use the instrument. The Scope is Located at 682 Medical Sciences Bldg.  The Imaris workstation is in 587B Medical Sciences Building. 

This is a short clip of live cell imaging of an early developmental stage of fertilized Zebrafish embryo that was acquired with the Andor.  The microtubules are in red and the DNA is in cyan.

Andor Live Cell Imaging

Andor Revolution XD Confocal inverted microscope

  • Nikon Objectives:
    • 100x Apo TIRF (Oil, NA = 1.49, WD = 0.12 mm) with Temperature Correction collar (for 23°C and 37°C)
    • 60x Plan Apo VC (Oil, NA = 1.4, WD = 0.13 mm)
    • 40x Plan Fluor (Oil, NA = 1.3, WD = 0.2 mm)
    • 20x Plan Fluor (Dry, NA = 0.5, WD = 2.1 mm)
  • Andor Laser Combiner (ALC): Four solid state lasers at 405, 488, 561, and 640 nm with 10 millisecond switch time and active blanking control to minimize unnecessary illumination.
  • Confocal emission bandpass filters:
    • DAPI: 447/60 (417-477nm)
    • GFP: 525/30 (510-540nm)
    • RFP: 607/36 (589-625nm)
    • Far-red (CY5): 685/40 (665-705nm)
  • Okolab CO2 cage incubator: Environmental regulation of temperature, CO2, and humidity to achieve cell culture conditions.  Temperature is thermostat-regulated to ± 0.1°C from 3° above ambient temperature up to 50°C.
  • Bitplane’s Imaris Imaging Workstation for advanced data display and analysis.  Microscope users may train themselves on the software and will receive free software use based on their microscope use.

Supported by the Department of Ophthalmology and Visual Sciences, the University of Wisconsin School of Medicine & Public Health, the Carl and Mildred Reeves Foundation for Macular Degeneration Research, and generous funding provided by the Schuette/Kraemer Family gift.

For questions and to schedule, contact:

Hongyu Noel, Core Instrument Manager
561A Med Sci
1300 University Ave.
Tel: 608-262-8055