Equipment

GUIDELINES FOR USE OF CORE EQUIPMENT DURING COVID19

• Even though we do not have COVID restrictions, please be mindful of disinfecting equipment.  The virus is still circulating.
• Materials (Disinfecting wipes or 70% ethanol) to disinfect the equipment/computers are available in each room.  Please spray ethanol on a towel and wipe off items vs. spraying the ethanol directly on the equipment.
• Here is a good article on properly sanitizing a microscope, that can be applied to other equipment.

Core users who have filled out a Vision Core Services Request Form have access to the following equipment:

Medical Sciences Center (Bardeen, SMI, Medical Sciences and Medical Sciences Center Complex) – 1300 University Avenue

Reserve time on the Zeiss Scopes or Imaris and Zen analysis workstations (trained users only) : FOM calendar and tracking software (please contact Inna Larsen for account setup).   Other equipment (MSC Licor and ERG) have Outlook reservation calendars.  Please contact Inna Larsen to add your email to the calendars. All Zeiss microscope users MUST be trained by Mark Filla before working on their own.

• Andor Revolution XD spinning disk confocal microscope (inverted) – live cell imaging and Imaris analysis workstation for images
• Zeiss Axioimager Z2 upright microscope for brightfield and epifluorescence microscopy.
• Zeiss PALM Microbeam Laser Capture Microdissection (LCM) system laser microdissection and analysis for DNA, RNA and protein isolation from live cells and slides
• Zeiss Axiovert inverted microscope for brightfield and epifluorescence microscopy
• Zen analysis workstation for the Zeiss inverted and upright scopes
• Biotek Neo2 Synergy plate reader can perform absorbance, fluorescence, luminescence.
• QuantStudio 7 Flex Real-Time PCR System
• LiCor Odyssey CLx uses near-infrared fluorescence to provide sensitivity equal or better than chemiluminescence giving you low background, high signal-to-noise, multiplexed detection, and the sensitivity of any fluorescent system. The Odyssey CLx System can be used for many applications, including Western blot analysis, EMSA, protein arrays, In-Cell Western™ Assays, On Cell Western Assays, Nucleic acid gel documentation, in vivo imaging, Coomassie gel documentation, RNAi analysis, and tissue section analysis.
• Luminex MAGPIX multiplex analyzer  can detect up to 50 cytokine/chemokine/growth factor/mRNA per sample in one hour.  Kits are commercially available from ThermoFisher and Millipore. For RNA expression assay RNA multiplexing products such as QuantiGene (system overview here) offer RNA probes for more than 25,000+ validated genes and can quantify gene expression in % change. Lysates can be used in checking RNA expression with no need of RNA purification or cDNA synthesis.
• Diagnosys Celeris Rodent ERG System this system is capable of Full–field ERG/VEP and Pattern ERG/VEP, Frequency series, Flash VEP, Scotopic threshold ERG
• Roland Consult combined electroretinography/imaging unit This system for full-field white and color flash/flicker ERG/VEP and multifocal ERG/cSLO/AF. This system can be used in rodents, dogs and cats
• Heidelberg Spectralis HRA + OCT provides multi-modality imaging of the eye using a spectral-domain OCT integrated with confocal scanning laser ophthalmoscopy. Multi-modality imaging offers insights into structure, function and metabolic activity of the retina. The Spectralis provides point-to-point correlation between fundus and OCT scans, producing an unmatched overall perspective of the retina and optic nerve head.
• Phenosys qOMR provides automated quantitative assessment of visual acuity and sensitivity based on optomotor responses in awake, unrestrained mice
• Agilent SeaHorse HS Mini Analyzer measures and reports energy metabolism and vital indicators of mitochondrial health, toxicity, and overall cellular function.
• TopCon Slitlamp SLD-701  Five magnifications, infra-red capability for meibography capture and the blue-free filter system for fluorescent observation

Clinical Sciences Center (600 Highland Ave)  and WIMR  (1111 Highland Ave.)

• WIMR Optical Imaging Core located on Floor 2 of the Wisconsin Institutes for Medical Research, offers imaging services on three microscopes systems, (1) a Leica SP8 3X STED Super-resolution microscope, (2) Nikon A1RS confocal microscope, and (3) Leica DMi8 inverted fluorescent microscope. In addition to training and usage on these systems, we offer many companion imaging and prep services. Powerful computing equipment is also available for advanced image processing and analysis.
• Heidelberg Spectralis HRA + OCT provides multi-modality imaging of the eye using a spectral-domain OCT integrated with confocal scanning laser ophthalmoscopy. Multi-modality imaging offers insights into structure, function and metabolic activity of the retina. The Spectralis provides point-to-point correlation between fundus and OCT scans, producing an unmatched overall perspective of the retina and optic nerve head.
• LiCor Odyssey CLx uses near-infrared fluorescence to provide sensitivity equal or better than chemiluminescence giving you low background, high signal-to-noise, multiplexed detection, and the sensitivity of any fluorescent system. The Odyssey CLx System can be used for many applications, including Western blot analysis, EMSA, protein arrays, In-Cell Western™ Assays, On Cell Western Assays, Nucleic acid gel documentation, in vivo imaging, Coomassie gel documentation, RNAi analysis, and tissue section analysis.
• Zeiss OMPI Lumera Operating Microscope and image capture system for ocular surgical procedures in animals
• Zeiss Axioimager Z2 with apotome: upright microscope for brightfield and epifluorescence microscopy
• Zeiss Axiovert inverted microscope for brightfield and epifluorescence microscopy. Has a live cell imaging chamber.
• Logos Biosystems X-Clarity II Tissue Clearing Processor – Embeds tissues in a hydrogel matrix and extracts lipids to create a transparent tissue-hydrogel hybrid that can be labeled with antibodies and imaged.
• Fundus camera, Electrophysiology equipment for mfERG, ffERG, PERG, PVEP, slit lamps, organ culture system