Equipment and Calendar

Core users who have filled out a Vision Core Request For Services Form have access to the following equipment:

Medical Sciences Center (Bardeen, SMI, Medical Sciences and Medical Sciences Center Complex) – 1300 University Avenue

  • Andor Revolution XD spinning disk confocal microscope (inverted).  Trained users can reserve scope time on the calendar. Please see the Usage and Rates page for fee info.
  • Zeiss Axioimager Z2  upright microscope for brightfield and ephttp://sites.ophth.wisc.edu/core/equipment/andor/ifluorescence microscopy
  • Zeiss Axiovert inverted microscope for brightfield and epifluorescence microscopy
  • Olympus BX40 brightfield microscope with an Olympus DP26 5 megapixel color camera and Olympus cellSens digital imaging software. Together, this allows the user to acquire high resolution (2448 x 1920 pixels) color images of histochemically or immunohistochemically stained sections, cells or whole mounts.  10X, 20X, and 40X air objectives along with a 100X oil objective are included with the microscope.
  • Biotek Neo2 Synergy plate reader can do absorbance, fluorescence, luminescence.
  • Applied Biosystems Real Time PCR Machines – QuantStudio 7 Flex Real-Time PCRSystem and ABI7300
  • LiCor Odyssey CLx uses near-infrared fluorescence to provide sensitivity equal or better than chemiluminescence giving you low background, high signal-to-noise, multiplexed detection, and the sensitivity of any fluorescent system.  The Odyssey CLx System can be used for many applications, including Western blot analysisEMSAprotein arraysIn-Cell Western™ AssaysOn Cell Western AssaysNucleic acid gel documentationin vivoimagingCoomassie gel documentationRNAi analysis, and tissue section analysis.
  • Luminex MAGPIX multiplex analyzer from Sigma Millipore. This machine detects up to 50 different proteins in a single reaction volume and reads a 96-well-plate in just 60 minutes.
  • Heidelberg Spectralis HRA + OCT ® provides multi-modality imaging of the eye using a spectral-domain OCT integrated with confocal scanning laser ophthalmoscopy (cSLO).  Multi-modality imaging offers insights into structure, function and metabolic activity of the retina. The Spectralis provides point-to-point correlation between fundus and OCT scans, producing an unmatched overall perspective of the retina and optic nerve head. Spectralis® imaging modalities include:

    The Spectralis is used widely in clinics and clinical trials. Visit Heidelberg Engineering’s website for more details.  Reserve time on the calendar.

 

Clinical Sciences Center – 600 Highland Ave and WIMR II – 1111  Highland Ave

  • Zeiss Axiophot microscope for light and fluorescence microscopy
  • Fundus camera
  • Electrophysiology equipment for mfERG, ffERG, PERG, PVEP
  • Optical coherence tomographer
  • Ultrasound biomicroscope
  • Fluorotron master fluorophotometer
  • Sonometrics SBR-300 A-scan ultrasonograph
  • Slit lamps with Goldmann applanation tonometer
  • Hartinger coincidence refractometers
  • Keeler instruments indirect ophthalmoscope
  • Zeiss Op-Mi 6 operating microscope
  • WIMR Optical Imaging Core located on Floor 2 of the Wisconsin Institutes for Medical Research, offers imaging services on three microscopes systems, (1) a Leica SP8 3X STED Super-resolution microscope, (2) Nikon A1RS confocal microscope, and (3) Leica DMi8 inverted fluorescent microscope. In addition to training and usage on these systems, we offer many companion imaging and prep services. Powerful computing equipment is also available for advanced image processing and analysis.